4M48

X-ray structure of dopamine transporter elucidates antidepressant mechanism

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
IMGT Antibody Annotation
SAbDab Antibody Annotation
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD
Membrane Protein Annotation: mpstruc

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C [auth H]	d4m48h_	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	V set domains (antibody variable domain-like)	automated matches	(Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
B [auth L]	d4m48l1	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	automated matches	automated matches	(Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C [auth H]	C1-set_1	e4m48H1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_1	ECOD (1.6)
C [auth H]	V-set_10	e4m48H2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set_10	ECOD (1.6)
A	SNF	e4m48A1	A: alpha complex topology	X: Sodium:neurotransmitter symporter family (SNF)-like (From Topology)	H: Sodium:neurotransmitter symporter family (SNF)-like (From Topology)	T: Sodium:neurotransmitter symporter family (SNF)-like	F: SNF	ECOD (1.6)
B [auth L]	C1-set_4	e4m48L1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_4	ECOD (1.6)
B [auth L]	V-set	e4m48L2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C [auth H]	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
B [auth L]	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)

IMGT Antibody Annotation IMGT Database Homepage

Chain	Protein Name	Description	Organism Name	Gene Allele Name(s)	Domain Name(s)	Receptor Type	Receptor Description	Provenance Source
C [auth H]	9D5 Fab	VH-CH1	Mus musculus (house mouse)	IGHV5-9-401, IGHJ201, IGHG2A01, IGHG2A02	V-DOMAIN VH, C-DOMAIN CH1	IG	FAB-GAMMA-2A_KAPPA	IMGT (202448-0)
B [auth L]	9D5 Fab	L-KAPPA	Mus musculus (house mouse)	IGKV4-57-101, IGKJ202, IGKC01, IGKC02	V-DOMAIN V-KAPPA, C-DOMAIN C-KAPPA	IG	FAB-GAMMA-2A_KAPPA	IMGT (202448-0)

SAbDab Antibody Annotation SAbDab Database Homepage

Chain	Chain Class	Chain Subclass	Chain Type	Antigen Name(s)	Provenance Source
C [auth H]	Heavy Chain	IGHV5	-	transporter	SAbDab (2024-12-06)
B [auth L]	Light Chain	IGKV4	Kappa	transporter	SAbDab (2024-12-06)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00209	Sodium:neurotransmitter symporter family (SNF)	Sodium:neurotransmitter symporter family	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
C [auth H]	9D5 antibody, heavy chain	-	-	-
A	Transporter	monoatomic cation transmembrane transporter activity monoatomic anion transmembrane transporter activity chloride transmembrane transporter activity solute:sodium symporter activity solute:monoatomic cation symporter activity organic hydroxy compound transmembrane transporter activity monoatomic anion:monoatomic cation symporter activity secondary active transmembrane transporter activity active monoatomic ion transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity transporter activity monoatomic anion:sodium symporter activity monoamine transmembrane transporter activity monoatomic cation transmembrane transporter activity monoatomic anion transmembrane transporter activity chloride transmembrane transporter activity solute:sodium symporter activity solute:monoatomic cation symporter activity organic hydroxy compound transmembrane transporter activity monoatomic anion:monoatomic cation symporter activity secondary active transmembrane transporter activity active monoatomic ion transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity transporter activity monoatomic anion:sodium symporter activity monoamine transmembrane transporter activity sodium ion transmembrane transporter activity sodium:chloride symporter activity chloride:monoatomic cation symporter activity active transmembrane transporter activity monoatomic ion transmembrane transporter activity metal ion transmembrane transporter activity transmembrane transporter activity symporter activity dopamine:sodium symporter activity inorganic anion transmembrane transporter activity cation binding cocaine binding binding ion binding organic cyclic compound binding heterocyclic compound binding small molecule binding metal ion binding	response to stimulus response to chemical response to odorant multicellular organismal process sleep adult locomotory behavior behavior adult behavior locomotory behavior amino acid transport localization transport establishment of localization inorganic ion homeostasis response to stimulus response to chemical response to odorant multicellular organismal process sleep adult locomotory behavior behavior adult behavior locomotory behavior amino acid transport localization transport establishment of localization inorganic ion homeostasis intracellular calcium ion homeostasis homeostatic process calcium ion homeostasis regulation of cytosolic calcium ion concentration regulation of presynaptic cytosolic calcium ion concentration cellular homeostasis neuron cellular homeostasis chemical homeostasis intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis monoamine transport norepinephrine transport nitrogen compound transport catecholamine transport organic hydroxy compound transport organic cation transport import into cell cellular localization signaling cell communication dopamine transport catecholamine uptake dopamine uptake involved in synaptic transmission synaptic signaling establishment of localization in cell neurotransmitter reuptake trans-synaptic signaling regulation of biological process catecholamine uptake involved in synaptic transmission biological regulation cell-cell signaling neurotransmitter transport chemical synaptic transmission anterograde trans-synaptic signaling cellular process neurotransmitter uptake synaptic transmission, dopaminergic dopamine uptake rhythmic process circadian sleep/wake cycle circadian behavior rhythmic behavior circadian rhythm monoatomic ion transmembrane transport sodium ion transmembrane transport inorganic ion transmembrane transport metal ion transport inorganic cation transmembrane transport transmembrane transport monoatomic cation transmembrane transport monoatomic ion transport monoatomic cation transport sodium ion transport	cell periphery cellular anatomical structure plasma membrane membrane presynapse synapse cell junction presynaptic membrane plasma membrane region synaptic membrane somatodendritic compartment neuronal cell body membrane cell body cell body membrane cell periphery cellular anatomical structure plasma membrane membrane presynapse synapse cell junction presynaptic membrane plasma membrane region synaptic membrane somatodendritic compartment neuronal cell body membrane cell body cell body membrane neuronal cell body axon cell projection neuron projection plasma membrane bounded cell projection
B [auth L]	9D5 antibody, light chain	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000175	Sodium:neurotransmitter symporter	Family
A	IPR037272	Sodium:neurotransmitter symporter superfamily	Homologous Superfamily

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A	OPM	Transmembrane	Alpha-helical polytopic	APC (Amino acid-Polyamine-organoCation) superfamily	Neurotransmitter: sodium symporter

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A	MemProtMD	Annotated as membrane protein by MemProtMD

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	Solute Carrier (SLC) Transporter Superfamily	DAT Dopamine transporter in complex with nortriptyline TCA (tricyclic antidepressant)

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.