Download MendeleySingle particle cryo-EM co-structure of E. coli AcrB with bound BDM91531 inhibitor at 3.23 A resolution
Boernsen, C., Mueller, R.T.To be published.
8QZQ
Single particle cryo-EM co-structure of E. coli AcrB with bound BDM91531 inhibitor
- PDB DOI: https://doi.org/10.2210/pdb8QZQ/pdb
- EM Map EMD-18780: EMDB EMDataResource
- Classification: TRANSPORT PROTEIN
- Organism(s): Escherichia coli K-12
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Membrane Protein: Yes PDBTM
- Deposited: 2023-10-29 Released: 2024-11-06
- Funding Organization(s): German Federal Ministry for Education and Research, Agence Nationale de la Recherche (ANR)
Experimental Data Snapshot
- Method: ELECTRON MICROSCOPY
- Resolution: 3.23 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Multidrug efflux pump subunit AcrB | 1,049 | Escherichia coli K-12 | Mutation(s): 0 Gene Names: acrB Membrane Entity: Yes |  | |
UniProt | |||||
Find proteins for P31224 (Escherichia coli (strain K12)) Explore P31224 Go to UniProtKB: P31224 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P31224 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| WR6 Query on WR6 | E [auth B] | [(2~{R})-1-[[(2~{R})-2,3-bis(oxidanyl)propoxy]-oxidanyl-phosphoryl]oxy-3-undecanoyloxy-propan-2-yl] tricosanoate C40 H79 O10 P QVTMHGKJALUQCM-XPSQVAKYSA-N |  | ||
| XE9 (Subject of Investigation/LOI) Query on XE9 | D [auth A] | [3-(3-chloranyl-2-piperazin-1-yl-quinolin-6-yl)phenyl]methanamine C20 H21 Cl N4 PMBAQAZUQBDHLD-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: ELECTRON MICROSCOPY
- Resolution: 3.23 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
| Task | Software Package | Version |
|---|---|---|
| RECONSTRUCTION | cryoSPARC | 4 |
Entry History & Funding Information
Deposition Data
- Released Date: 2024-11-06 Deposition Author(s): Boernsen, C., Mueller, R.T., Pos, K.M., Frangakis, A.S.
| Funding Organization | Location | Grant Number |
|---|---|---|
| German Federal Ministry for Education and Research | Germany | BMBF- 16GW0236K |
| Agence Nationale de la Recherche (ANR) | France | ANR-19-AMRB-0007 |
Revision History (Full details and data files)
- Version 1.0: 2024-11-06
Type: Initial release
