Annotations: 5H8O

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	V-set_8	e5h8oA1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set_8	ECOD (1.6)
B	CARD	e5h8oB1	A: alpha arrays	X: DEATH domain	H: DEATH domain (From Topology)	T: DEATH domain	F: CARD	ECOD (1.6)

SAbDab Antibody Annotation SAbDab Database Homepage

Chain	Chain Class	Chain Subclass	Chain Type	Antigen Name(s)	Provenance Source
A	Heavy Chain	IGHV1	-	apoptosis-associated speck-like protein containing a card	SAbDab (2024-12-06)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
B	PF00619	Caspase recruitment domain (CARD)	Caspase recruitment domain	Motif contained in proteins involved in apoptotic signaling. Predicted to possess a DEATH (Pfam:PF00531) domain-like fold .	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	VHH nanobody	-	-	-
B	Apoptosis-associated speck-like protein containing a CARD	protein binding binding protein dimerization activity enzyme binding protease binding BMP receptor binding receptor serine/threonine kinase binding signaling receptor binding transmembrane receptor protein serine/threonine kinase binding identical protein binding protein homodimerization activity cytoskeletal protein binding tropomyosin binding enzyme regulator activity protein binding binding protein dimerization activity enzyme binding protease binding BMP receptor binding receptor serine/threonine kinase binding signaling receptor binding transmembrane receptor protein serine/threonine kinase binding identical protein binding protein homodimerization activity cytoskeletal protein binding tropomyosin binding enzyme regulator activity enzyme activator activity peptidase regulator activity molecular function activator activity cysteine-type endopeptidase activator activity involved in apoptotic process molecular function regulator activity cysteine-type endopeptidase regulator activity involved in apoptotic process peptidase activator activity peptidase activator activity involved in apoptotic process Pyrin domain binding protein domain specific binding transmembrane transporter binding interleukin-6 receptor binding cytokine receptor binding growth factor receptor binding myosin I binding myosin binding	regulation of cellular process regulation of catalytic activity positive regulation of protein metabolic process positive regulation of peptidase activity positive regulation of biological process regulation of primary metabolic process positive regulation of molecular function positive regulation of metabolic process regulation of proteolysis proteolysis regulation of endopeptidase activity positive regulation of macromolecule metabolic process protein metabolic process positive regulation of cellular process regulation of cellular process regulation of catalytic activity positive regulation of protein metabolic process positive regulation of peptidase activity positive regulation of biological process regulation of primary metabolic process positive regulation of molecular function positive regulation of metabolic process regulation of proteolysis proteolysis regulation of endopeptidase activity positive regulation of macromolecule metabolic process protein metabolic process positive regulation of cellular process regulation of cysteine-type endopeptidase activity regulation of biological process regulation of protein metabolic process regulation of peptidase activity biological regulation positive regulation of catalytic activity primary metabolic process regulation of macromolecule metabolic process regulation of molecular function positive regulation of hydrolase activity positive regulation of cysteine-type endopeptidase activity regulation of hydrolase activity positive regulation of endopeptidase activity positive regulation of proteolysis metabolic process cellular process macromolecule metabolic process regulation of metabolic process activation of cysteine-type endopeptidase activity protein maturation zymogen activation gene expression macromolecule biosynthetic process biosynthetic process protein processing response to stimulus immune response regulation of adaptive immune response adaptive immune response positive regulation of response to stimulus regulation of response to stimulus positive regulation of immune response immune system process regulation of immune response regulation of immune system process positive regulation of adaptive immune response positive regulation of immune system process cellular response to stimulus signaling signal transduction by p53 class mediator cell death intracellular signal transduction cell communication apoptotic signaling pathway intrinsic apoptotic signaling pathway by p53 class mediator signal transduction apoptotic process intrinsic apoptotic signaling pathway programmed cell death regulation of signal transduction regulation of programmed cell death regulation of intrinsic apoptotic signaling pathway regulation of intracellular signal transduction regulation of signaling regulation of apoptotic process regulation of cell communication regulation of apoptotic signaling pathway positive regulation of DNA-binding transcription factor activity RNA metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process nucleobase-containing compound biosynthetic process nucleic acid metabolic process regulation of gene expression regulation of DNA-binding transcription factor activity regulation of macromolecule biosynthetic process regulation of DNA-templated transcription regulation of localization import into cell positive regulation of cellular component organization regulation of cellular component organization transport vesicle-mediated transport cellular component organization localization positive regulation of endocytosis phagocytosis cellular component organization or biogenesis regulation of endocytosis regulation of transport regulation of phagocytosis regulation of vesicle-mediated transport establishment of localization positive regulation of phagocytosis positive regulation of transport endocytosis multicellular organismal process positive regulation of interleukin-8 production positive regulation of macromolecule biosynthetic process positive regulation of biosynthetic process cytokine production regulation of cytokine production regulation of multicellular organismal process interleukin-8 production positive regulation of gene expression positive regulation of multicellular organismal process positive regulation of cytokine production regulation of interleukin-8 production response to osmotic stress cellular response to chemical stress cellular response to osmotic stress cellular response to environmental stimulus cellular response to chemical stimulus osmosensory signaling pathway cellular response to stress response to stress response to chemical response to abiotic stimulus cellular response to abiotic stimulus regulation of chemokine production chemokine production positive regulation of chemokine production interleukin-10 production regulation of interleukin-10 production positive regulation of interleukin-10 production macropinocytosis pinocytosis regulation of defense response regulation of response to biotic stimulus biological process involved in interspecies interaction between organisms regulation of response to external stimulus response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus regulation of defense response to virus defense response to virus defense response to other organism regulation of response to stress positive regulation of defense response to virus by host regulation of defense response to virus by host response to external biotic stimulus response to virus defense response to Gram-negative bacterium defense response to bacterium response to bacterium regulation of immune effector process positive regulation of myeloid leukocyte cytokine production involved in immune response positive regulation of immune effector process myeloid leukocyte cytokine production cytokine production involved in immune response production of molecular mediator of immune response regulation of cytokine production involved in immune response immune effector process regulation of macrophage cytokine production positive regulation of cytokine production involved in immune response positive regulation of production of molecular mediator of immune response regulation of production of molecular mediator of immune response positive regulation of macrophage cytokine production macrophage cytokine production response to peptide cellular response to interleukin-1 response to cytokine cellular response to cytokine stimulus response to interleukin-1 positive regulation of cell communication positive regulation of signal transduction positive regulation of canonical NF-kappaB signal transduction intracellular signaling cassette positive regulation of intracellular signal transduction canonical NF-kappaB signal transduction regulation of canonical NF-kappaB signal transduction positive regulation of signaling positive regulation of ERK1 and ERK2 cascade positive regulation of MAPK cascade regulation of MAPK cascade MAPK cascade ERK1 and ERK2 cascade regulation of ERK1 and ERK2 cascade extrinsic apoptotic signaling pathway positive regulation of apoptotic signaling pathway positive regulation of programmed cell death cell surface receptor signaling pathway positive regulation of extrinsic apoptotic signaling pathway positive regulation of apoptotic process regulation of extrinsic apoptotic signaling pathway negative regulation of biological process negative regulation of type I interferon production negative regulation of cellular process regulation of interferon-beta production interferon-beta production negative regulation of macromolecule metabolic process type I interferon production negative regulation of metabolic process negative regulation of cytokine production negative regulation of gene expression negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process negative regulation of interferon-beta production negative regulation of multicellular organismal process regulation of type I interferon production negative regulation of DNA-binding transcription factor activity negative regulation of NF-kappaB transcription factor activity negative regulation of molecular function cellular response to tumor necrosis factor response to tumor necrosis factor positive regulation of response to biotic stimulus pattern recognition receptor signaling pathway immune response-activating signaling pathway positive regulation of response to external stimulus immune response-regulating signaling pathway innate immune response innate immune response-activating signaling pathway positive regulation of innate immune response regulation of innate immune response positive regulation of defense response activation of innate immune response activation of immune response positive regulation of organelle organization regulation of cytoskeleton organization regulation of actin filament-based process regulation of cellular component size cellular component biogenesis regulation of protein-containing complex assembly positive regulation of protein-containing complex assembly cellular component assembly positive regulation of protein polymerization protein-containing complex assembly actin filament-based process regulation of actin filament polymerization regulation of biological quality protein polymerization actin polymerization or depolymerization regulation of actin filament organization actin filament polymerization actin cytoskeleton organization protein-containing complex organization positive regulation of cellular component biogenesis positive regulation of cytoskeleton organization actin filament organization regulation of actin polymerization or depolymerization positive regulation of supramolecular fiber organization regulation of cellular component biogenesis regulation of organelle organization organelle organization regulation of protein polymerization regulation of actin filament length regulation of actin cytoskeleton organization regulation of supramolecular fiber organization supramolecular fiber organization positive regulation of actin filament polymerization cytoskeleton organization regulation of anatomical structure size regulation of non-canonical NF-kappaB signal transduction non-canonical NF-kappaB signal transduction positive regulation of non-canonical NF-kappaB signal transduction myeloid dendritic cell activation cell activation myeloid leukocyte activation leukocyte activation regulation of tumor necrosis factor production positive regulation of tumor necrosis factor superfamily cytokine production tumor necrosis factor superfamily cytokine production positive regulation of tumor necrosis factor production tumor necrosis factor production regulation of tumor necrosis factor superfamily cytokine production protein complex oligomerization protein homooligomerization regulation of type II interferon production type II interferon production positive regulation of type II interferon production regulation of inflammatory response positive regulation of inflammatory response inflammatory response apoptotic mitochondrial changes regulation of release of cytochrome c from mitochondria positive regulation of release of cytochrome c from mitochondria mitochondrion organization release of cytochrome c from mitochondria regulation of mitochondrion organization interleukin-1 production regulation of interleukin-1 beta production positive regulation of interleukin-1 beta production positive regulation of interleukin-1 production regulation of interleukin-1 production interleukin-1 beta production positive regulation of JNK cascade JNK cascade regulation of JNK cascade regulation of protein stability positive regulation of NF-kappaB transcription factor activity inflammasome-mediated signaling pathway canonical inflammasome complex assembly NLRP3 inflammasome complex assembly cytoplasmic pattern recognition receptor signaling pathway intracellular receptor signaling pathway positive regulation of locomotion regulation of cell motility regulation of lymphocyte migration positive regulation of cell migration positive regulation of cell motility regulation of cell migration positive regulation of mononuclear cell migration cell migration cell motility regulation of mononuclear cell migration locomotion positive regulation of lymphocyte migration regulation of leukocyte migration positive regulation of T cell migration lymphocyte migration positive regulation of leukocyte migration leukocyte migration regulation of locomotion regulation of T cell migration mononuclear cell migration T cell migration cytokine-mediated signaling pathway tumor necrosis factor-mediated signaling pathway regulation of tumor necrosis factor-mediated signaling pathway regulation of cytokine-mediated signaling pathway regulation of response to cytokine stimulus interleukin-6 production positive regulation of interleukin-6 production regulation of interleukin-6 production negative regulation of signaling negative regulation of intracellular signal transduction negative regulation of canonical NF-kappaB signal transduction negative regulation of cell communication negative regulation of response to stimulus negative regulation of signal transduction response to lipid cellular response to lipopolysaccharide response to oxygen-containing compound cellular response to molecule of bacterial origin response to lipopolysaccharide cellular response to oxygen-containing compound cellular response to biotic stimulus cellular response to lipid response to molecule of bacterial origin icosanoid metabolic process small molecule biosynthetic process oxoacid metabolic process carboxylic acid metabolic process organic acid biosynthetic process organic acid metabolic process carboxylic acid biosynthetic process icosanoid biosynthetic process small molecule metabolic process positive regulation of cell population proliferation regulation of leukocyte activation regulation of cell activation positive regulation of cell activation T cell activation lymphocyte proliferation positive regulation of leukocyte activation regulation of leukocyte proliferation cell population proliferation positive regulation of T cell activation positive regulation of lymphocyte proliferation positive regulation of T cell proliferation positive regulation of activated T cell proliferation regulation of cell population proliferation mononuclear cell proliferation regulation of leukocyte cell-cell adhesion positive regulation of leukocyte proliferation regulation of activated T cell proliferation positive regulation of leukocyte cell-cell adhesion lymphocyte activation leukocyte proliferation leukocyte cell-cell adhesion activated T cell proliferation positive regulation of cell-cell adhesion positive regulation of lymphocyte activation cell adhesion cell-cell adhesion positive regulation of mononuclear cell proliferation regulation of cell-cell adhesion T cell proliferation regulation of lymphocyte proliferation positive regulation of cell adhesion regulation of cell adhesion regulation of T cell activation regulation of T cell proliferation regulation of mononuclear cell proliferation regulation of lymphocyte activation regulation of protein modification process regulation of protein phosphorylation negative regulation of phosphate metabolic process negative regulation of catalytic activity regulation of transferase activity negative regulation of kinase activity negative regulation of transferase activity regulation of phosphate metabolic process negative regulation of protein serine/threonine kinase activity phosphate-containing compound metabolic process negative regulation of protein modification process phosphorus metabolic process protein modification process negative regulation of protein metabolic process protein phosphorylation phosphorylation negative regulation of protein kinase activity regulation of protein kinase activity regulation of kinase activity negative regulation of protein phosphorylation macromolecule modification regulation of protein serine/threonine kinase activity negative regulation of phosphorus metabolic process regulation of phosphorylation regulation of phosphorus metabolic process negative regulation of phosphorylation positive regulation of antigen processing and presentation of peptide antigen positive regulation of antigen processing and presentation of peptide antigen via MHC class II positive regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II positive regulation of antigen processing and presentation antigen processing and presentation regulation of antigen processing and presentation antigen processing and presentation of peptide or polysaccharide antigen via MHC class II regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II regulation of antigen processing and presentation of peptide antigen regulation of antigen processing and presentation of peptide antigen via MHC class II antigen processing and presentation of peptide antigen via MHC class II antigen processing and presentation of peptide antigen intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator DNA damage response intrinsic apoptotic signaling pathway in response to DNA damage catabolic process autophagy regulation of catabolic process regulation of autophagy process utilizing autophagic mechanism myeloid dendritic cell activation involved in immune response cell activation involved in immune response myeloid cell activation involved in immune response leukocyte activation involved in immune response pyroptotic inflammatory response defense response to Gram-positive bacterium regulation of cytokine production involved in inflammatory response negative regulation of cytokine production involved in inflammatory response production of molecular mediator involved in inflammatory response cytokine production involved in inflammatory response	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol canonical inflammasome complex protein-containing complex NLRP6 inflammasome complex membrane-bounded organelle Golgi membrane Golgi apparatus membrane intracellular membrane-bounded organelle endomembrane system intracellular organelle cellular anatomical structure intracellular anatomical structure cytoplasm cytosol canonical inflammasome complex protein-containing complex NLRP6 inflammasome complex membrane-bounded organelle Golgi membrane Golgi apparatus membrane intracellular membrane-bounded organelle endomembrane system intracellular organelle bounding membrane of organelle organelle organelle membrane nucleus membrane-enclosed lumen intracellular membraneless organelle nucleolus intracellular organelle lumen organelle lumen membraneless organelle nuclear lumen extracellular region vesicle lumen intracellular vesicle cytoplasmic vesicle lumen secretory granule lumen cytoplasmic vesicle secretory granule secretory vesicle vesicle catalytic complex IkappaB kinase complex transferase complex protein kinase complex transferase complex, transferring phosphorus-containing groups serine/threonine protein kinase complex NLRP3 inflammasome complex AIM2 inflammasome complex somatodendritic compartment cell body neuronal cell body polymeric cytoskeletal fiber cytoskeleton supramolecular fiber microtubule supramolecular polymer supramolecular complex microtubule cytoskeleton mitochondrion lytic vacuole vacuolar lumen lysosome azurophil granule vacuole azurophil granule lumen primary lysosome nucleoplasm NLRP1 inflammasome complex endoplasmic reticulum

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
B	IPR051249	NLRP Inflammasome	Family
B	IPR011029	Death-like domain superfamily	Homologous Superfamily
B	IPR004020	DAPIN domain	Domain
B	IPR033516	CARD8/ASC/NALP1, CARD domain	Domain
B	IPR001315	CARD domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
B	Pharos: Q9ULZ3	large cell carcinoma malignant mesothelioma squamous cell carcinoma heart valve disease tubular adenocarcinoma hyperhomocysteinemia adenocarcinoma Albuminuria non-small cell lung carcinoma kidney disease calcinosis colorectal cancer granular cell carcinoma subependymal giant cell astrocytoma lung benign neoplasm large cell carcinoma malignant mesothelioma squamous cell carcinoma heart valve disease tubular adenocarcinoma hyperhomocysteinemia adenocarcinoma Albuminuria non-small cell lung carcinoma kidney disease calcinosis colorectal cancer granular cell carcinoma subependymal giant cell astrocytoma lung benign neoplasm malignant glioma cancer nephrosclerosis colorectal carcinoma ovarian cancer cribriform carcinoma of breast pilocytic astrocytoma gastric neoplasm small cell lung carcinoma Graves disease systemic lupus erythematosus hepatocellular carcinoma invasive ductal breast carcinoma lung cancer breast cancer malignant pancreatic neoplasm pancreatic ductal adenocarcinoma ductal breast carcinoma in situ psoriasis glioblastoma Hashimoto thyroiditis tuberculosis hereditary diffuse gastric adenocarcinoma lung carcinoma lymphoplasmacytic lymphoma osteosarcoma cribriform carcinoma pancreatic intraductal papillary-mucinous carcinoma gastric cancer small cell carcinoma

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.