3HXZ

Crystal Structure of catalytic fragment of E. coli AlaRS G237A in complex with AlaSA

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	tRNA-synt_2c_2nd	e3hxzA1	A: alpha complex topology	X: Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS) (From Topology)	H: Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS) (From Topology)	T: Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)	F: tRNA-synt_2c_2nd	ECOD (1.6)
A	tRNA-synt_2c_1st	e3hxzA2	A: a+b three layers	X: Class II aaRS and biotin synthetases (From Topology)	H: Class II aaRS and biotin synthetases (From Topology)	T: Class II aaRS and biotin synthetases	F: tRNA-synt_2c_1st	ECOD (1.6)
B	tRNA-synt_2c_2nd	e3hxzB1	A: alpha complex topology	X: Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS) (From Topology)	H: Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS) (From Topology)	T: Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)	F: tRNA-synt_2c_2nd	ECOD (1.6)
B	tRNA-synt_2c_1st	e3hxzB2	A: a+b three layers	X: Class II aaRS and biotin synthetases (From Topology)	H: Class II aaRS and biotin synthetases (From Topology)	T: Class II aaRS and biotin synthetases	F: tRNA-synt_2c_1st	ECOD (1.6)
D	tRNA-synt_2c_2nd	e3hxzD1	A: alpha complex topology	X: Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS) (From Topology)	H: Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS) (From Topology)	T: Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)	F: tRNA-synt_2c_2nd	ECOD (1.6)
D	tRNA-synt_2c_1st	e3hxzD2	A: a+b three layers	X: Class II aaRS and biotin synthetases (From Topology)	H: Class II aaRS and biotin synthetases (From Topology)	T: Class II aaRS and biotin synthetases	F: tRNA-synt_2c_1st	ECOD (1.6)
C	tRNA-synt_2c_2nd	e3hxzC1	A: alpha complex topology	X: Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS) (From Topology)	H: Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS) (From Topology)	T: Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)	F: tRNA-synt_2c_2nd	ECOD (1.6)
C	tRNA-synt_2c_1st	e3hxzC2	A: a+b three layers	X: Class II aaRS and biotin synthetases (From Topology)	H: Class II aaRS and biotin synthetases (From Topology)	T: Class II aaRS and biotin synthetases	F: tRNA-synt_2c_1st	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.930.10	Alpha Beta	2-Layer Sandwich	BirA Bifunctional Protein	domain 2	CATH (4.3.0)
B	3.30.930.10	Alpha Beta	2-Layer Sandwich	BirA Bifunctional Protein	domain 2	CATH (4.3.0)
D	3.30.930.10	Alpha Beta	2-Layer Sandwich	BirA Bifunctional Protein	domain 2	CATH (4.3.0)
C	3.30.930.10	Alpha Beta	2-Layer Sandwich	BirA Bifunctional Protein	domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF01411	tRNA synthetases class II (A) (tRNA-synt_2c)	tRNA synthetases class II (A)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Alanyl-tRNA synthetase	RNA binding binding organic cyclic compound binding tRNA binding nucleic acid binding transcription regulator activity DNA-binding transcription repressor activity DNA-binding transcription factor activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding RNA binding binding organic cyclic compound binding tRNA binding nucleic acid binding transcription regulator activity DNA-binding transcription repressor activity DNA-binding transcription factor activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding identical protein binding protein binding protein homodimerization activity protein dimerization activity Ser-tRNA(Ala) hydrolase activity hydrolase activity, acting on ester bonds catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid carboxylic ester hydrolase activity aminoacyl-tRNA editing activity hydrolase activity catalytic activity, acting on a tRNA catalytic activity ligase activity, forming carbon-oxygen bonds alanine-tRNA ligase activity aminoacyl-tRNA ligase activity ligase activity cation binding transition metal ion binding zinc ion binding metal ion binding ligase activity, forming phosphoric ester bonds catalytic activity, acting on a protein peptide lactyltransferase (ATP-dependent) activity	regulation of cellular process negative regulation of biological process RNA metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process regulation of RNA metabolic process negative regulation of cellular process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of cellular process negative regulation of biological process RNA metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process regulation of RNA metabolic process negative regulation of cellular process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process negative regulation of DNA-templated transcription regulation of biological process negative regulation of metabolic process biological regulation primary metabolic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of gene expression biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process metabolic process cellular process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process negative regulation of RNA metabolic process amino acid activation tRNA aminoacylation amino acid metabolic process translation tRNA aminoacylation for protein translation tRNA metabolic process alanyl-tRNA aminoacylation protein metabolic process	cellular anatomical structure membrane intracellular anatomical structure cytoplasm cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR045864	Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL)	Homologous Superfamily
A, B, C, D	IPR018164	Alanyl-tRNA synthetase, class IIc, N-terminal	Domain
A, B, C, D	IPR018162	Alanine-tRNA ligase, class IIc, anti-codon-binding domain superfamily	Homologous Superfamily
A, B, C, D	IPR012947	Threonyl/alanyl tRNA synthetase, SAD	Domain
A, B, C, D	IPR009000	Translation protein, beta-barrel domain superfamily	Homologous Superfamily
A, B, C, D	IPR023033	Alanine-tRNA ligase, eukaryota/bacteria	Family
A, B, C, D	IPR003156	DHHA1 domain	Domain
A, B, C, D	IPR002318	Alanine-tRNA ligase, class IIc	Family
A, B, C, D	IPR050058	Alanine--tRNA ligase	Family
A, B, C, D	IPR018163	Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily	Homologous Superfamily
A, B, C, D	IPR018165	Alanyl-tRNA synthetase, class IIc, core domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.